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中国癌症防治杂志

中国癌症防治杂志 ›› 2011, Vol. 3 ›› Issue (3): 196-200.doi: 10.3969/j.issn.1674-5671.2011.03.04

• 基础研究 • 上一篇    下一篇

CO2对宫颈癌Hela细胞CDK9基因表达的影响

潘灵辉, 林飞, 李力, 黄冰   

  1. 南宁广西医科大学附属肿瘤医院麻醉科；妇瘤科
  • 出版日期:2011-09-25 发布日期:2011-10-17
  • 基金资助:

    广西壮族自治区自然科学基金资助项目（桂科基 0575088）

CO2-insufflation on expression of CDK9 mRNA of cervical cancer cell Hela

PAN Ling-Hui, LIN Fei, LI Li, HUANG Bing   

  • Online:2011-09-25 Published:2011-10-17

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摘要： 目的 研究CO2对宫颈癌Hela细胞株CDK9基因表达的影响，分析其在癌细胞生长与转移中的作用。方法 将宫颈癌Hela细胞株随机分为3组：A组Hela细胞株在压力为8mmHg的纯CO2下通气4h，培养24h；B组Hela细胞株在压力为8mmHg的纯CO2下通气4h，培养120h；C组Hela细胞株未进行CO2处理，细胞株置于常规细胞培养箱中培养120h。采用RT-PCR法检测A、B、C组宫颈癌Hela细胞CDK9 mRNA的表达。结果 A、B组与C组比较，A、B组CDK9 mRNA的相对表达量明显下调（P＜0.05）；B组与A组比较，B组CDK9 mRNA的相对表达量比A组下调更显著（P＜0.01）。结论 宫颈癌Hela细胞CDK9基因在CO2作用下受抑制出现表达下调，且随CO2作用时间越长，CDK9基因相对表达量降低越明显。

关键词: RT-PCR, 细胞周期依赖性激酶9, 宫颈癌细胞, 基因表达

Abstract: Objective To investigate the effect of CO2-insufflation on expression of CDK9 mRNA of cervical cancer cell line Hela, and to analyze the role of CO2 in growth and metastasis of Hela. Methods Hela cells were randomly divided into two study groups (A and B) and one control group (C). The cells in group A were exposed to CO2 with 8 mmHg and incubated for 24 hours,the cells in group B were exposed to CO2 with 8 mmHg and incubated for 120 hours, the cells in group C were not exposed to CO2.Real-time PCR was used to detect the expression of CDK9 mRNA of Hela cells collected from each group.ResultsT he expression of CDK9 mRNA of Hela cells in the two study groups was lower than that in control group (P＜0.05),and the expression of CDK9 mRNA of Hela cells in group B was lower than that in group A (P＜0.01)Conclusions The expression of CDK9 mRNA can be down-regulated by CO2-insufflation.The expression of CDK9 mRNA decreased more obvious with longer incubation time

Key words: RT-PCR;, CDK9, Cervical cancer cells, Gene expression